Recent publications

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Evaluating the Performance of Machine Learning Approaches to Predict the Microbial Quality of Surface Waters and to Optimize the Sampling Effort
Manel Naloufi, Françoise S Lucas, Sami Souihi, Pierre Servais, Aurélie Janne, Thiago Wanderley Matos de Abreu
, 2021, 13, ⟨10.3390/w13182457⟩
Heavy Metal Accumulation and Changes in Soil Enzymes Activities and Bacterial Functional Diversity under Long-Term Treated Wastewater Irrigation in East Central Region of Tunisia (Monastir Governorate)
Marouane Mkhinini, Iteb Boughattas, Vanessa Alphonse, Alexandre Livet, Stéphanie Gıustı-Mıller, Mohamed Bannı, Noureddine Bousserrhıne
, 2020, 235, pp.106150. ⟨10.1016/j.agwat.2020.106150⟩
Effect of Aloe Vera Wastes on Physico-Chemical Properties and Microbiological Activity in Soils
Fatma Lanouar, Iteb Boughattas, Marouene Mkhinini, Vanessa Alphonse, Stephanie Gustier-Muller, Alex Livet, Mohamed Banni, Nourreddine Bousserhine
, 2018, 3 (4), pp.1292--1304. ⟨10.22161/ijeab/3.4.21⟩
Influence of Ecological Restoration on Mercury Mobility and Microbial Activities on Former Guyanese Mining Sites
Ewan Couic, Vanessa Alphonse, Alexandre Livet, Stéphanie Giusti-Miller, Noureddine Bousserrhine
, 2021, 11 (5), pp.2231. ⟨10.3390/app11052231⟩
Use of Earthworms Eisenia Andrei on the Bioremediation of Contaminated Area in North of Tunisia and Microbial Soil Enzymes as Bioindicator of Change on Heavy Metals Speciation
Iteb Boughattas, Sabrine Hattab, Vanessa Alphonse, Alexandre Livet, Stéphanie Giusti-Miller, Hamadi Boussetta, Mohamed Banni, Noureddine Bousserrhine
, 2019, 19 (1), pp.296--309. ⟨10.1007/s11368-018-2038-8⟩

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Offre de stage de M2 au Leesu et au CERTES en 2023 : microbial proliferation on raw earth materials

by Julien Le Roux - published on

Le sujet de stage concerne l’évaluation des cinétiques de développement microbiens dans les matériaux en terre crue en fonction des conditions environnementales et des caractéristiques du matériau, afin d’évaluer les risques potentiels pour l’environnement intérieur et les conditions d’utilisation de ces matériaux.

Contexte scientifique du stage

Buildings of the future are called to meet increasingly high-performance requirements and to ensure adequate comfort for residents. Most of the countries all over the world cope with issues related to the amount of waste production, energy consumption and carbon dioxide emissions. These issues have stimulated a keen interest in the application of geo and bio-based materials in construction and in the rediscovery of some ancient constructive techniques based on their use. Among these, raw earth material has proved to be particularly interesting for its intrinsic availability, sustainability, and recyclability. Technically, those materials provide very interesting thermal and hydric properties.

Use of raw earth-based materials is currently hindered by the lack of specific legislation regulating its conformity to safety standards in terms of internal air quality for example. The increase of relative humidity in building interior can indeed lead to the proliferation of microorganisms and a release of several harmful compounds, such as volatile organic compounds, toxins, parietal compounds or spores, which may induce health issues for inhabitants. Little is currently known about the risks of microbial growth associated with the use of raw earth materials in construction. Further characterization is therefore necessary to know the behavior of these materials in the different contexts of use and environmental conditions.

Objectifs du stage

This internship topic aims to set up and perform different methods to characterize microbial proliferation on raw earth construction materials. These approaches will be based on conventional culture, optical microscopy and molecular biology techniques (qPCR). The quantification and identification of microorganisms present on the surface and inside the raw earth materials will be carried out for samples subjected to different humidity (water vapor, liquid water) and temperature conditions. These results will then be put into perspective with the use of different formulations and methods of preparation of the raw earth samples. Finally, potential solid particles caused by this phenomenon and suspended in the air would be collected and analyzed to assess the impact of such incidents on internal air quality. Thus, it will be possible to determine the conditions of use and risk for these materials.

Conditions du stage et de dépôt de candidature

Internship dates: The internship will start at the beginning of February 2023 and will last 6 months.

Workplace: The internship will mainly take place at the IUT Sénart-Fontainebleau as well as the LEESU and CERTES laboratories (Créteil & Moissy-Cramayel, France).

Contacts :
Alexis Simon

Recruitment: Send a resume and a cover letter to Alexis Simons: alexis.simons[at] ; and Hamza Allam: allam.hamza[at] An interview will be organized.

Offre de stage complète : fichier PDF